University of Isfahan Journal of Plant Biological Sciences 3041-9603 2 3 2010 05 22 Comparative study of tropane alkaloids production in hairy roots and plantlet cultures of Atropa belladonna L. by salicylic acid treatments Comparative study of tropane alkaloids production in hairy roots and plantlet cultures of Atropa belladonna L. by salicylic acid treatments 63 76 18773 FA Najmeh Ahmadian Chashmi Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University of Tehran, Iran Mozafar Sharifi Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University of Tehran, Iran Farah Karimi Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran Hasan Rahnama Agricultural Biotechnology Research Institute, Seed and Plant Improvement Institute, Tehran, Iran Journal Article 2016 06 14 Most Solanaceae plants produce a range of biologically important alkaloids including nicotine and tropane alkaloids, such as hyoscyamine (atropine) and scopolamine. These alkaloids are used for their medicinal properties. Atropa belladonna is a medicinally important herbaceous plant that produces high amount of tropane alkaloids in roots. In this research, Atropa belladonna explants were obtained from sterilized seedlings in the modified Murashige and Skoog (MS) solid medium. The seeds were collected from Vaz and Garmestan regions of Mazandaran Province, Iran. Explants were cultured for four weeks on a modified MS solid medium, containing different concentrations of salicylic acid (0, 0.01, 0.1 and 1 mM). In addition, hairy roots of Atropa belladonna that was established by transformation with Agrobacterium rhizogenes, were cultured in MS medium for two weeks. Then the production of two tropane alkaloids, atropine and scopolamine, in different parts of neoformed plants were assayed by HPLC method. Generally, tropan alkaloids content in hairy roots was considerably higher compared to those contents in plants. Although Garmestan population was better than Vaz group, the amount of atropine in hairy roots was influenced by salicylic acid as high as 5 to 35 times of produced atropine in different parts of plants. The scopolamine content in the hairy roots was 2-30 times higher than in plant organs. In conclusion, it is suggested that, hairy root lines can be used as a replacement of plants in further studies and for tropane alkaloids production in economical and commercial scales. Most Solanaceae plants produce a range of biologically important alkaloids including nicotine and tropane alkaloids, such as hyoscyamine (atropine) and scopolamine. These alkaloids are used for their medicinal properties. Atropa belladonna is a medicinally important herbaceous plant that produces high amount of tropane alkaloids in roots. In this research, Atropa belladonna explants were obtained from sterilized seedlings in the modified Murashige and Skoog (MS) solid medium. The seeds were collected from Vaz and Garmestan regions of Mazandaran Province, Iran. Explants were cultured for four weeks on a modified MS solid medium, containing different concentrations of salicylic acid (0, 0.01, 0.1 and 1 mM). In addition, hairy roots of Atropa belladonna that was established by transformation with Agrobacterium rhizogenes, were cultured in MS medium for two weeks. Then the production of two tropane alkaloids, atropine and scopolamine, in different parts of neoformed plants were assayed by HPLC method. Generally, tropan alkaloids content in hairy roots was considerably higher compared to those contents in plants. Although Garmestan population was better than Vaz group, the amount of atropine in hairy roots was influenced by salicylic acid as high as 5 to 35 times of produced atropine in different parts of plants. The scopolamine content in the hairy roots was 2-30 times higher than in plant organs. In conclusion, it is suggested that, hairy root lines can be used as a replacement of plants in further studies and for tropane alkaloids production in economical and commercial scales. https://ijpb.ui.ac.ir/article_18773_d39204fbb6a01b5867d38f3711cfb0de.pdf