University of Isfahan Journal of Plant Biological Sciences 3041-9603 2 4 2010 08 23 Chemical variation of Astragalus verus Olivier (Fabaceae) according to flavonoid pattern in the West of Iran Chemical variation of Astragalus verus Olivier (Fabaceae) according to flavonoid pattern in the West of Iran 67 80 18782 FA Mahtab Asgari Nematian Department of Biology, Payame Noor University, Hamedan Morteza Atri Department of Biology, Faculty of Sciences, Bu-Ali Sina University, Hamedan Abdolkarime Chehregani Rad Department of Biology, Faculty of Sciences, Bu-Ali Sina University, Hamedan Journal Article 2016 06 14 Astragalus is the largest genus of vascular plants. This genus has 2500-3000 species and 250 sections. In regard to economic and medicinal importance of Astragalus genus, this study, was carried out to determine and to distinguish of intraspecific diversity in phenolic components of Astragalus verus in the west of Iran. For this reason, leave phenolic components belonging to 20 different stations were extracted and analyzed by TLC (Thin Layer Chromatography), to examine the presence or absence of three flavonoidic standards, such as flavones, quercetine and rutin. Finally, data of flavonoid studies were analyzed by MVSP (Multivariate Statistical Package) and SPSS (Statistical Package for the Social Science) softwares with WARD, PCA (Principle Component Analysis) and UPGMA (Unweighted Pair Group Method with Arithmetic mean) methods. Results of flavonoid studies showed intraspecific diversity in chemical components (chemotype) level. The results showed different phenolic groups of Astragalus verus from different stations with different ecological conditions. The studied ecological factors included soil texture, pH, EC, elevation, latitude and longitude. Ecological data analysis showed that, among studied ecological factors, elevation from sea level has the most important role in observed intraspecisic diversity of Astragalus verus in the west of Iran. Astragalus is the largest genus of vascular plants. This genus has 2500-3000 species and 250 sections. In regard to economic and medicinal importance of Astragalus genus, this study, was carried out to determine and to distinguish of intraspecific diversity in phenolic components of Astragalus verus in the west of Iran. For this reason, leave phenolic components belonging to 20 different stations were extracted and analyzed by TLC (Thin Layer Chromatography), to examine the presence or absence of three flavonoidic standards, such as flavones, quercetine and rutin. Finally, data of flavonoid studies were analyzed by MVSP (Multivariate Statistical Package) and SPSS (Statistical Package for the Social Science) softwares with WARD, PCA (Principle Component Analysis) and UPGMA (Unweighted Pair Group Method with Arithmetic mean) methods. Results of flavonoid studies showed intraspecific diversity in chemical components (chemotype) level. The results showed different phenolic groups of Astragalus verus from different stations with different ecological conditions. The studied ecological factors included soil texture, pH, EC, elevation, latitude and longitude. Ecological data analysis showed that, among studied ecological factors, elevation from sea level has the most important role in observed intraspecisic diversity of Astragalus verus in the west of Iran. https://ijpb.ui.ac.ir/article_18782_27691ad5484aaab44a790f2ff6f6f163.pdf