University of Isfahan Journal of Plant Biological Sciences 3041-9603 3 9 2011 11 22 Morphology of the shoot apical meristem and its effects on the explants viability in grapevine Morphology of the shoot apical meristem and its effects on the explants viability in grapevine 27 38 18819 FA Gholam Reza Gohari Department of Horticultural Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Somayeh Faridi Nicharan Department of Horticultural Science, Faculty of Agriculture, University of Urmieh, Urmieh, Iran Somayeh Naghiloo Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Alireza Mottalebi Azar Department of Horticultural Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Mohammad Reza Dadpour Department of Horticultural Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran Journal Article 2016 06 14 Shoot apical meristem (SAM) culture is known as an effective method for the production of virus free plants. Detection and then dissection of SAM are the first two steps in establishment of successful culture. Providing of leaf primordium-free meristem is a very difficult operation due to the small size and colorless appearance of the SAM. Amongst the commercial plants, grapevine demonstrates specific and complex developmental pattern at which the SAM plays a crucial role. In this research, the live meristems were stained using novel procedure and then they were stained by water solution of FDA (0.1%) and fuchsine acid (0.05%), simultaneously. The prepared meristems were then detected and excised under a fluorescence stereomicroscope (510 nm) and afterwards they were immediately cultured on the half strength MS medium. Microscopic studies were shown that the size of SAM in the grapevine was fluctuated in terms of its developmental course. The higher organogenesis ability of SAM, the more viability of the explants that were achieved during the meristem culture. On the other hand, viability of meristem containing leaf and uncommitted primordium was higher than that of organ-free meristem. Shoot apical meristem (SAM) culture is known as an effective method for the production of virus free plants. Detection and then dissection of SAM are the first two steps in establishment of successful culture. Providing of leaf primordium-free meristem is a very difficult operation due to the small size and colorless appearance of the SAM. Amongst the commercial plants, grapevine demonstrates specific and complex developmental pattern at which the SAM plays a crucial role. In this research, the live meristems were stained using novel procedure and then they were stained by water solution of FDA (0.1%) and fuchsine acid (0.05%), simultaneously. The prepared meristems were then detected and excised under a fluorescence stereomicroscope (510 nm) and afterwards they were immediately cultured on the half strength MS medium. Microscopic studies were shown that the size of SAM in the grapevine was fluctuated in terms of its developmental course. The higher organogenesis ability of SAM, the more viability of the explants that were achieved during the meristem culture. On the other hand, viability of meristem containing leaf and uncommitted primordium was higher than that of organ-free meristem. https://ijpb.ui.ac.ir/article_18819_bbe84c521db3e8e845a22f661412441c.pdf