University of Isfahan Journal of Plant Biological Sciences 3041-9603 4 11 2012 04 20 Enhancement of lignan and phenylpropanoid compounds production by chitosan in Linum album cell culture Enhancement of lignan and phenylpropanoid compounds production by chitosan in Linum album cell culture 13 26 18834 FA Sedigheh Esmaeilzadeh Bahabadi Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran Department of Plant Science, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Mozafar Sharifi Department of Plant Science, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Naser Safaie Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran Mehrdad Behmanesh Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Journal Article 2016 06 14 Podophyllotoxin (PTOX) is a lignan compound which occurs in a few plant species and has pharmacological significance for its anticancer activities. Linum album, one of endemic species in Iran, has PTOX and other lignans. Chitin and Chitosan are the main compounds of fungal species and as biotic elicitors could be used to improve secondary metabolites. In this study, we investigated the different concentration effects of chitin and chitosan on cell growth, PTOX and lariciresinol production in l. album cell culture. Next, we evaluated the effecr of optimal concentration of chitosan on lignan, phenol, flavonoid, flavonol and lignin content at different times. Treatment of L. album cell cultures with the 100 mg/L chitosan increased the production of PTOX and lariciresinol about two times higher than contol. In addition, Chitosan increased phenol, flavonoid and flavonol. Lignin was increased 2-fold higher than the control in response to chitosan. To study mechanism of chitosan action, phenylalanine ammonio-lyase (PAL) and cinnamyl alcohol dehydrogenase (CAD) activity were investigated. The activity of PAL and CAD enzymes involved in the first steps of the PTOX biosynthesis was activated by chitosan, reaching a peak within three days after treatment. Chitosan regulated the production of PTOX, lariciresinol and phenylpropanoid compounds by effecting on enzymes activity through PTOX biosynthesis pathway. Podophyllotoxin (PTOX) is a lignan compound which occurs in a few plant species and has pharmacological significance for its anticancer activities. Linum album, one of endemic species in Iran, has PTOX and other lignans. Chitin and Chitosan are the main compounds of fungal species and as biotic elicitors could be used to improve secondary metabolites. In this study, we investigated the different concentration effects of chitin and chitosan on cell growth, PTOX and lariciresinol production in l. album cell culture. Next, we evaluated the effecr of optimal concentration of chitosan on lignan, phenol, flavonoid, flavonol and lignin content at different times. Treatment of L. album cell cultures with the 100 mg/L chitosan increased the production of PTOX and lariciresinol about two times higher than contol. In addition, Chitosan increased phenol, flavonoid and flavonol. Lignin was increased 2-fold higher than the control in response to chitosan. To study mechanism of chitosan action, phenylalanine ammonio-lyase (PAL) and cinnamyl alcohol dehydrogenase (CAD) activity were investigated. The activity of PAL and CAD enzymes involved in the first steps of the PTOX biosynthesis was activated by chitosan, reaching a peak within three days after treatment. Chitosan regulated the production of PTOX, lariciresinol and phenylpropanoid compounds by effecting on enzymes activity through PTOX biosynthesis pathway. https://ijpb.ui.ac.ir/article_18834_649402416d0253f73e5c9a9d3f16e1d3.pdf