University of Isfahan Journal of Plant Biological Sciences 3041-9603 4 14 2013 02 19 OsABI5 and OsVP1, two transcription factors involved in abiotic stress response in rice OsABI5 and OsVP1, two transcription factors involved in abiotic stress response in rice 49 60 18866 FA Maryam-Sadat Shobbar Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran Zahra-Sadat Shobbar Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran Vahid Niknam Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran Journal Article 2016 06 14 Rice (Oryza sativa L.) which is the main food for more than half of the world’s population, is often considered as one of the most drought, salt and cold-sensitive crop plants particularly at early stages of post germination. Plants monitor the environmental status during a post-germination growth arrest and this prevents them to grow under unfavourable conditions which ultimately helps them to survive. In Arabidopsis, this response is mediated by abscisic acid and requires the ABI5 and ABI3 transcription factors. In this research, we investigated whether a similar abiotic stress induced growth arrest mechanism was operating in rice seedlings through the expression analysis of OsVP1 and OsABI5 (rice orthologues of the Arabidopsis transcription factors ABI3 and ABI5) in two rice cultivars, FL478 and IR29, which were salt tolerant and sensitive respectively. For exposing to salt, drought or cold stresses, the two-day seedlings were transferred to MS media complemented with iso-osmotic concentrations of NaCl (0, 50, 100 and 150 mM) or mannitol (0, 100, 180 and 275 mM) at 25°C or MS media at 4 and 15°C for 10 days. In both cultivars, stresses decreased shoot growth and increased the transcript level of OsABI5 and OsVP1 genes. Therefore, based on the achieved results, probably, OsABI5 and OsVP1 transcription factors either directly or indirectly regulated the expression of other genes involved in abiotic stress-induced growth arrest in rice. Rice (Oryza sativa L.) which is the main food for more than half of the world’s population, is often considered as one of the most drought, salt and cold-sensitive crop plants particularly at early stages of post germination. Plants monitor the environmental status during a post-germination growth arrest and this prevents them to grow under unfavourable conditions which ultimately helps them to survive. In Arabidopsis, this response is mediated by abscisic acid and requires the ABI5 and ABI3 transcription factors. In this research, we investigated whether a similar abiotic stress induced growth arrest mechanism was operating in rice seedlings through the expression analysis of OsVP1 and OsABI5 (rice orthologues of the Arabidopsis transcription factors ABI3 and ABI5) in two rice cultivars, FL478 and IR29, which were salt tolerant and sensitive respectively. For exposing to salt, drought or cold stresses, the two-day seedlings were transferred to MS media complemented with iso-osmotic concentrations of NaCl (0, 50, 100 and 150 mM) or mannitol (0, 100, 180 and 275 mM) at 25°C or MS media at 4 and 15°C for 10 days. In both cultivars, stresses decreased shoot growth and increased the transcript level of OsABI5 and OsVP1 genes. Therefore, based on the achieved results, probably, OsABI5 and OsVP1 transcription factors either directly or indirectly regulated the expression of other genes involved in abiotic stress-induced growth arrest in rice. https://ijpb.ui.ac.ir/article_18866_bd5a5a19f12b90d6e80614f49be41eb3.pdf