University of Isfahan Journal of Plant Biological Sciences 3041-9603 7 23 2015 03 21 Production of transgenic soybean by the half-seed explant Production of transgenic soybean by the half-seed explant 29 40 18961 FA Somayeh Hooshyar Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran Mohammad Bagher Bagherieh-Najjar Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran Mahnaz Aghdasi Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran Ahmad Abdolzadeh Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran Journal Article 2016 06 14 oybean (Glycine max), one of the most important agronomic crops in the world, is considered as a rich source of oil and protein production and much effort has been directed towards its genetic improvements using conventional breeding techniques, as well as molecular genetic approaches. Meanwhile, optimization of transformation of soybean requires an efficient system for production of stable transgenic lines. In this study, we reported the first attempt to use the cotyledonary node derived from mature seed for Agrobacterium mediate plant transformation in Iran. Surface-sterilized soybean seeds of DPX and Gorgan3 cultivars were soaked in distilled water overnight and used for explant production. The explants were inoculated in Agrobacterium tumefaciense (LBA4404) containing pBI121 solution. Positive transgenic plants which remained green in the presence of kanamycin, as a selectable marker, were used for the GUS assay. PCR, sequencing and the GUS assay showed successful expression of the GUS gene in the regenerated plants in both cultivars. Simplicity and efficiency were the main advantages of using the half-seed method which can be used to introduce useful and/or novel gene(s) into soybean. oybean (Glycine max), one of the most important agronomic crops in the world, is considered as a rich source of oil and protein production and much effort has been directed towards its genetic improvements using conventional breeding techniques, as well as molecular genetic approaches. Meanwhile, optimization of transformation of soybean requires an efficient system for production of stable transgenic lines. In this study, we reported the first attempt to use the cotyledonary node derived from mature seed for Agrobacterium mediate plant transformation in Iran. Surface-sterilized soybean seeds of DPX and Gorgan3 cultivars were soaked in distilled water overnight and used for explant production. The explants were inoculated in Agrobacterium tumefaciense (LBA4404) containing pBI121 solution. Positive transgenic plants which remained green in the presence of kanamycin, as a selectable marker, were used for the GUS assay. PCR, sequencing and the GUS assay showed successful expression of the GUS gene in the regenerated plants in both cultivars. Simplicity and efficiency were the main advantages of using the half-seed method which can be used to introduce useful and/or novel gene(s) into soybean.

https://ijpb.ui.ac.ir/article_18961_f3038123452f855e081067492eaa0abf.pdf