University of Isfahan Journal of Plant Biological Sciences 3041-9603 7 24 2015 06 22 Effect of explant origin and different growth regulators on micropropagation of Pistacia atlantica ssp. mutica Effect of explant origin and different growth regulators on micropropagation of Pistacia atlantica ssp. mutica 67 76 18965 FA Zeinab Safari Department of Agronomy and Plant Breeding, Faculty of Horticulture, Ilam University, Ilam, Iran Ali-Ashraf Mehrabi Department of Agronomy and Plant Breeding, Faculty of Horticulture, Ilam University, Ilam, Iran Ali Arminian Department of Agronomy and Plant Breeding, Faculty of Horticulture, Ilam University, Ilam, Iran Journal Article 2016 06 14 Propagation of wild pistachio as a multipurpose woody species is a hard and tedious task. In this research, an effective in vitro protocol was developed for rapid proliferation of wild pistachio (Pistacia atlantica ssp. mutica) in MS medium supplemented with B5 vitamins and different growth regulators. Rooting of plantlets was tested by two treatments containing Rhizopon and IBA in ex vitro. With respect to the results, the nodal segments explants, produced the highest shoot frequency, leaf frequency and the tallest shoots. On the other hand, the tallest shoots were generated from shoot tip explant and medium containing of TDZ plus IAA. Both treatments (Rhizopon and IBA) led to a remarkable increase in the number of roots, root length and rooting percentage compared to the control. These results may be applied for rapid proliferation to spread the pistachio trees and shrubs that are difficult and time consuming. Propagation of wild pistachio as a multipurpose woody species is a hard and tedious task. In this research, an effective in vitro protocol was developed for rapid proliferation of wild pistachio (Pistacia atlantica ssp. mutica) in MS medium supplemented with B5 vitamins and different growth regulators. Rooting of plantlets was tested by two treatments containing Rhizopon and IBA in ex vitro. With respect to the results, the nodal segments explants, produced the highest shoot frequency, leaf frequency and the tallest shoots. On the other hand, the tallest shoots were generated from shoot tip explant and medium containing of TDZ plus IAA. Both treatments (Rhizopon and IBA) led to a remarkable increase in the number of roots, root length and rooting percentage compared to the control. These results may be applied for rapid proliferation to spread the pistachio trees and shrubs that are difficult and time consuming. https://ijpb.ui.ac.ir/article_18965_6ec3e7fda9e4848ecbe393f07167f433.pdf