University of Isfahan Journal of Plant Biological Sciences 3041-9603 7 26 2015 12 22 Induction of defensive responses and production of secondary metabolites in Linum album cell culture by ultrasound Induction of defensive responses and production of secondary metabolites in Linum album cell culture by ultrasound 27 40 18980 FA Armaghan Abedzadeh Neyshabouri Faculty of Agriculture, Islamic Azad University, Damghan Branch, Damghan, Iran Ayatollah Rezaei Department of Agricultural Biotechnology, Faculty of Agriculture, Shahed University, Tehran, Iran Journal Article 2016 06 14 Ultrasound (US) with low intensity is considered as a physical tool to stimulate living systems in medicine and biotechnology. This study aim to survey the US effects on some physiological parameters and podophyllotoxin production in Linum album cell culture. The cell cultures were treated with US waves with a frequency of 40 kHz and the power 5 Wcm-3, in a time dependent experiment with 3 replicates and the cells were collected at 24, 48 and 72 h after the treatment. Evaluated parameters were cell growth, contents of total protein, MDA, hydrogen peroxide, phenolics, flavonoids, activity of peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase and podophyllotoxin. The results showed that cell growth was significantly decreased by the US, but there was no significant change in protein content. The amount of hydrogen peroxide, membrane lipid peroxidation and activity of peroxidase, polyphenol oxidase and phenylalanine ammonia-lyase enzymes were increased in cells under the effect of US, compared to control. Also US increased the production of phenolic compounds, flavonoids and podophyllotoxin in treated cells. Maximum amount of podophyllotoxin was obtained in treated cultures after 72 hours which was the 268.9 µg/g DW (2.7 times that of the control). It seems that US by stimulating cells and induction of defensive responses and secondary metabolism increased the amount of podophyllotoxin in cells of Linum album plant. Ultrasound (US) with low intensity is considered as a physical tool to stimulate living systems in medicine and biotechnology. This study aim to survey the US effects on some physiological parameters and podophyllotoxin production in Linum album cell culture. The cell cultures were treated with US waves with a frequency of 40 kHz and the power 5 Wcm-3, in a time dependent experiment with 3 replicates and the cells were collected at 24, 48 and 72 h after the treatment. Evaluated parameters were cell growth, contents of total protein, MDA, hydrogen peroxide, phenolics, flavonoids, activity of peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase and podophyllotoxin. The results showed that cell growth was significantly decreased by the US, but there was no significant change in protein content. The amount of hydrogen peroxide, membrane lipid peroxidation and activity of peroxidase, polyphenol oxidase and phenylalanine ammonia-lyase enzymes were increased in cells under the effect of US, compared to control. Also US increased the production of phenolic compounds, flavonoids and podophyllotoxin in treated cells. Maximum amount of podophyllotoxin was obtained in treated cultures after 72 hours which was the 268.9 µg/g DW (2.7 times that of the control). It seems that US by stimulating cells and induction of defensive responses and secondary metabolism increased the amount of podophyllotoxin in cells of Linum album plant. https://ijpb.ui.ac.ir/article_18980_9f9ffc9d3c05ab59c02f5688d5cbda5b.pdf