University of Isfahan Journal of Plant Biological Sciences 3041-9603 10 1 2018 08 05 Effect of salicylic acid and methyl jasmonate on the production of rosmarinic acid and caffeic acid in callus culture of Salvia lerrifolia Benth Effect of salicylic acid and methyl jasmonate on the production of rosmarinic acid and caffeic acid in callus culture of Salvia lerrifolia Benth 67 80 22786 10.22108/ijpb.2018.108819.1071 FA Ziba Ghasimi Hagh Department of Horticulture science and plant protection, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran Somayyeh Jokar Department of Horticulture science and plant protection, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran Hojatollah Bodaghi Department of Horticulture science and plant protection, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran Masoomeh Modarres Department of Biology, Faculty of Science, Shahid Hasheminegad Campus, Farhangian University, Mashhad, Iran Journal Article 2018 01 01 In this study, callus formation of <em>Salvia leriifolia</em> Benth by stem and leaf explants in the combination of NAA and 2,4-D with Kin and the effect of salicylic acid and methyl jasmonate elicitors (50, 100, 150 mM) in callus on some secondary metabolites were studied. NAA in combination with Kin had no callus production in   both of stem and leaf explants, while the most appropriate callus produced by leaf explant at a concentration of 1 mg/L Kin with 2 mg/L 2,4-D. The highest fresh and dry weight, total phenol and flavonoids content were observed in calluses treated with 100 μM of methylisammonate, however, with increasing salicylic acid concentration, fresh and dry weight, total phenol content, flavonoids, rosmarinic acid increased, and the highest content of rusamicin acid and caffeic acid was observed in calluses treated with 100 and 150 μM salicylic acid, respectively. According to the results, it is stated that, by optimizing the concentrations of the ellicetors, it is possible to produce the desired secondary metabolites of salvia in <em>in vitro</em> conditions In this study, callus formation of <em>Salvia leriifolia</em> Benth by stem and leaf explants in the combination of NAA and 2,4-D with Kin and the effect of salicylic acid and methyl jasmonate elicitors (50, 100, 150 mM) in callus on some secondary metabolites were studied. NAA in combination with Kin had no callus production in   both of stem and leaf explants, while the most appropriate callus produced by leaf explant at a concentration of 1 mg/L Kin with 2 mg/L 2,4-D. The highest fresh and dry weight, total phenol and flavonoids content were observed in calluses treated with 100 μM of methylisammonate, however, with increasing salicylic acid concentration, fresh and dry weight, total phenol content, flavonoids, rosmarinic acid increased, and the highest content of rusamicin acid and caffeic acid was observed in calluses treated with 100 and 150 μM salicylic acid, respectively. According to the results, it is stated that, by optimizing the concentrations of the ellicetors, it is possible to produce the desired secondary metabolites of salvia in <em>in vitro</em> conditions https://ijpb.ui.ac.ir/article_22786_047abf77c6092247283cef5ecff40a10.pdf