نویسندگان
1 مرکز تحقیقات سلولی مولکولی، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران
2 بخش به زراعی مؤسسه تحقیقات و آموزش توسعه نیشکر خوزستان، اهواز، ایران
3 گروه زیستشناسی، دانشکده علوم، دانشگاه شهید چمران اهواز، اهواز، ایران
4 گروه بیوشیمی و سلولی مولکولی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران
چکیده
کلیدواژهها
عنوان مقاله [English]
نویسندگان [English]
Phenylpropanoid compounds as secondary metabolites are synthesized by plants during development and in response to stress. Phenylpropanoids contribute to the compounds of plant pigmentation, antioxidants and protective agents and also play a regulatory role in growth and morphogenesis. In this research, samples of blade and sheath of leaf, , stem, and root of Saccharum officinarum (cultivar CP69-1062) during the germination, tillering, grand growth, and maturation stages were prepared. In order to studying biochemical changes of phenylpropanoid compounds during development of sugarcane, PAL activity enzyme, phenolic, flavonoids and flavonols component in samples were analyzed. The study of changes of enzyme activity in different tissues during developmental stages showed high enzyme activity of PAL in leaves during all of developmental stages and in sheath during grand growth stage. Enzyme activity was increased in stem during grand growth stage and in root during germination stage. Results indicated that changes of concentration of total phenol and the maximum amount of total phenol were detected in leaf during germination and maturation stages, in sheath during germination stage, in stem during grand growth stage and in root during maturation stage (p<0.05). The maximum amount of flavonoids and flavonols were observed significantly in leaves during all of developmental stages. This variation may originate by the existing heterogeneity in different parts of plants and also chemical and biochemical features of this compound. In conclusion, PAL, as a rate-limiting enzyme of the phenylpropanoid pathway, displays critical role in the development of sugarcane, particularly in lignified tissues.
کلیدواژهها [English]