نوع مقاله : مقاله پژوهشی
نویسندگان
1 بررسی اثر محیط کشت، منبع آهن (FeEDHHA) و بیوراکتور بر ریزازدیادی پایه رویشی CAB 11E
2 گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه خوارزمی، تهران، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
The CAB11E rootstock is one of the cherry and sour cherry rootstocks introduced into the country recently. This study aimed to optimize the micropropagation of this rootstock. During the shoot multiplication stage, virus-free CAB 11E plantlets were cultured in a factorial experiment based on a completely randomized design using three basal culture media (MS, WPM, and DKW) and four hormone combinations, including 0.5, 1, and 2 mg L-1 BA, and a combination of 1 mg L-1 BA and 0.1 mg L-1 IBA. The results showed that MS medium supplemented with 1 mg L-1 BA and 0.1 mg L-1 IBA was the most effective, resulting in a multiplication rate of 4.4 and a shoot length of 3.5 cm. To evaluate the effect of the bioreactor system on shoot proliferation, plantlets were cultured in the optimal medium from the previous stage as liquid culture in a bioreactor. The results demonstrated that the multiplication rate in the bioreactor system increased to 14.5. For rooting, plantlets were cultured on MS basal medium containing different iron sources, full and half-strength macronutrients, and varying concentrations of IBA. In the optimal rooting treatment, the use of MS medium supplemented with Fe-EDDHA, half-strength macronutrients, and 0.1 mg L-1 IBA resulted in 97% rooting of the plantlets. These findings suggest that utilizing a bioreactor system, combined with the optimization of culture medium composition and iron source, can offer an efficient approach for micropropagating the CAB11E rootstock.
کلیدواژهها [English]
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